【文献标题】Sphk1 participates in malignant progression of breast cancer by regulating epithelial-mesenchymal transition and stem cell characteristics

【作者】Zhijie Chen, Bingxiong Liu

【作者单位】汉川市人民医院（Hanchuan People’s Hospital）

【文献中引用产品】

霍乱毒素 CAS号：9012-63-9

【关键词】Breast cancer，Sphingosine kinase 1，Epithelial-mesenchymal transition，Stem cell，Metastasis

【DOI】https://doi.org/10.1016/j.tice.2020.101380

【影响因子(IF)】2.90

【出版期刊】《Tissue and Cell》

【产品原文引用】

Cell and culture

Human breast epithelial cells (MCF-10A, CRL-10317) and BC cell lines MCF-7 (CRL-3435), T47D (HTB-133), SKBR3 (HTB-30), MDA-MB-231 (HTB-26), BT-474 (HTB-20) were purchased from American Tissue Culture Collection. The cells, except MDA-MB-23 cells, were cultured in DMEM (12491015, ThermoFisher, USA) containing 10 % FBS (30067334, ThermoFisher, USA), 1% penicillin/streptomycin (15140163,ThermoFisher, USA) at 37 °C in 5% CO2. MCF-10A cell line was cultured with additional 100 ng/mL cholera toxin (Hengyuan,China, http://www.hyswbio.com) on the basis of other cell culture condition. After culture for one day, the original medium was discarded, and the cells were washed 1–2 times by PBS. Next, 5 mL fresh DMEM medium was added and further incubated with the cells in a CO2 incubator. When the confluence reached 80%–90%, the cells were subcultured. MDA-MB-231 cells were cultured in Leibovitz’s L15 medium (11415064, ThermoFisher, USA) without CO2。

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