【文献标题】Transcriptome Characterization of Cymbidium sinense'Dharma' Using 454 Pyrosequencing and Its Application in the Identification of Genes Associated with Leaf Color Variation
【作者】Genfa Zhu，Fengxi Yang，Shanshan Shi，et.al
【作者单位】华中农业大学（Huazhong Agricultural University）
【文献中引用产品】
【DOI】DOI:10.1371/journal.pone.0128592
【影响因子(IF)】3.02
【出版期刊】《PLOS ONE》
【产品原文引用】
Biochemical measurements
For Chlorophyll content measurement, six-month old leaves of wild type and the mutant grown under identical conditions were collected and Chl (a,b) were extracted in 90% acetone and determined spectrophotometrically by the method of Lorenzen [26]. For the contents of chlorophyll intermediate products, leaves were homogenized in nine volume of 0.01 M Phosphate Buffered Saline (PBS), and mixed on the ice and centrifuged (30 min at 2,500g). The supernatants was assayed by a ELISA kit (HengYuan Biological Technology Co.,Ltd, Shanghai,China) separately. Data were analyzed by SPSS software.
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