【文献标题】Nitrogen Metabolism in Acorus calamus L. Leaves Induced Changes in Response to Microcystin–LR at Environmentally Relevant Concentrations

【作者】Guoyuan Chen，Qingsong Li，Mingxian Bai，et.al

【作者单位】厦门理工大学（Xiamen University of Technology）

【文献中引用产品】

微囊藻毒素LR（MC-LR）ELISA试剂盒

【关键词】Microcystin-leucine-arginine，Environmentally relevant concentrations，Acorus calamus Linnaeus，Nitrogen metabolism

【DOI】https://doi.org/10.1007/s00128-019-02597-y

【影响因子(IF)】2.9

【出版期刊】《Bulletin of Environmental Contamination and Toxicology》

【产品原文引用】

Acorus calamus L. plants with 10–12 cm long apical shoots were purchased from a local nursery (Xiamen, Fujian, China). They were then washed clean with deionized water and flushed three times with sterile water. Finally, each plant was inserted into a hole in a foam board with one foam board bearing three A. calamus L. shoots. The boards were then separately placed in a glass cylinder with 2L of 1/4 Hoagland nutritive solution (pH 6.5) and pre-cultured for 5 days. Subsequently, the nutritive solution was replaced with 1/4 Hoagland nutritive solution containing different concentrations of MC–LR (0 μg/L as the control group, and 1.0, 9.9, and 29.8 μg/L as treatment groups). The pure MC-LR (purity≥96%) was purchased from Alexis Biochemicals (Lufelfingen, Switzerland). The MC–LR concentrations in the nutritive solution were determined using enzyme linked immunoassay kit (detection limit 20ng/L) (ShangHai HengYuan Biological Technology Co., Ltd China). 

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