【文献标题】Evidence-based antioxidant activity of the essential oil from Fructus A. zerumbet on cultured human umbilical vein endothelial cells’ injury induced by ox-LDL

【作者】Xiang-chun Shen，Ling Tao，Wan-kui Li，et.al

【作者单位】贵阳医学院（Guiyang Medical University）

【文献中引用产品】

科研人血清

【关键词】Essential oil, Fructus Alpiniae zerumbet, Human umbilical vein endothelial cells, Oxidized low-density lipoprotein, Oxidative stress

【DOI】doi:10.1186/1472-6882-12-174

【影响因子(IF)】1.9

【出版期刊】《BMC Complementary Medicine and Therapies》

【产品原文引用】

Methods

Chemical and herbal materials

The culture medium was used M199 with Earle’s salts (Sigma-Aldrich), containing 20% (v/v) human serum (Shanghai Hengyuan, Shanghai, China), 5 U/ml penicillin G, 5 pg/ml streptomycin sulfate and 150 pg/ml endothelial cell growth supplement (Sigma-Aldrich).Cells reached confluency within approximately 4 d, and were cultured at 37°C in a humidified atmosphere with 5% CO2. The medium was changed a day after seeding and every 2 d thereafter.

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