【文献标题】Exposure to the environmental pollutant ammonia causes changes in gut microbiota and inflammatory markers in fattening pigs

【作者】Yutao Li， Runxiang Zhang，Xiang Liet，et.al

【作者单位】东北农业大学（Northeast Agricultural University）

【文献中引用产品】

猪基质金属蛋白酶-9（MMP-9）ELISA试剂盒

猪髓过氧化物酶（MPO）ELISA试剂盒

猪乳铁蛋白(LTF)ELISA试剂盒

猪钙卫蛋白（calprotein）ELISA试剂盒

【关键词】Ammonia，L-Selenomethionine，Gut microbiota，Inflammatory markers，Livestocks 

【DOI】https://doi.org/10.1016/j.ecoenv.2020.111564 

【影响因子(IF)】6.2

【出版期刊】《Ecotoxicology and Environmental Safety 》

【产品原文引用】

2.2. Detection of inflammatory marker levels in feces The collected fecal samples were diluted with cold physiological saline at a ratio of 1:9 and then centrifuged at 3,500 rpm for 10 min at 4 ℃. The supernatant was used for the subsequent analysis of inflammatory marker levels. The contents of Matrix metalloproteinase-9 (MMP-9), Myeloperoxidase (MPO), Lactoferrin (LTF) and Calprotectin were detected by corresponding ELISA assay kits (Shanghai Hengyuan biological technology Co., Ltd., Shanghai, China). All procedure was followed by the instructions of the manufacturer. The absorbance values were determined by the spectrophotometer (SpectraMax? ABS00254, California, USA). The maximum coefficient of variation presented a good reproducibility. Then, OD values were measured by enzymelabeled instrument at 450 nm. The relative contents of all inflammatory markers were calculated based on corresponding formulas. 

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