【文献标题】MicroRNA‐194 reduces inflammatory response and human dermal microvascular endothelial cells permeability
through suppression of TGF‐β/SMAD pathway by inhibiting THBS1 in chronic idiopathic urticaria
【作者】Shengming Qu | Lei Yang | Zhe Liu
【作者单位】吉林大学第二大医院（The Second Hospital of Jilin University）
【文献中引用产品】
生物素标记山羊抗兔二抗
【关键词】chronic idiopathic urticaria, human dermal microvascular endothelial cells permeability,inflammatory response, mast cells, mircoRNA‐194, TGF‐β/SMAD pathway, thrombospondin
【DOI】 10.1002/jcb.28941
【影响因子(IF)】8.9
【出版期刊】《Wiley》
【产品原文引用】
Parts of skin tissue of the subjects were collected, fixed by 4% paraformaldehyde, dehydrated by gradient ethanol and normal butanol and sliced into 5 μm sections. Afterward, the sections were supplemented with 100 μL 5% bovine serum albumin, 100 μL primary antibody THBS1 (1:200; ab93653; Abcam Inc., Cambridge, MA) and biotin‐labeled goat anti‐rabbit secondary antibody working solution (1:100; HY90046; Hengyuan Biological Technology Co Ltd, Shanghai, China), and streptomycin anti biotin peroxidase solution (Zhongshan Biotechnology Co, Beijing, China).
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